Catalog Number - 85518-05P

The 85518‑05P Cogent RP C18 ABX™ HPLC Column is engineered for durable, high‑performance separations under extreme mobile phase conditions, providing reliable operation across a wide pH range (2–12).

Built with a bulky, specialized end‑capped C18 phase bonded to high‑purity silica, this column minimizes secondary interactions and enhances peak shape for acidic, basic, hydrophobic, and neutral analytes, including compounds with very high or very low pKa values.

✅ Ideal as a USP L1 alternate for demanding methods involving strong acids or bases

50 mm Length – Fast Scouting & Method Development

• Designed for rapid separations and short run times

• Ideal for method development and screening workflows

• Reduces:

  • Solvent consumption

  • Run time per injection

✅ Frequently used as a scouting column for preparative method development

4.6 mm ID – Standard Analytical Platform

• Compatible with standard HPLC systems

• Ideal for:

  • routine QC analysis

  • method development and optimization

  • scale‑up preparation

Typical Flow Rate Range

• ~0.8–1.5 mL/min

✅ Provides robust and reproducible analytical performance

5 µm Particle – Maximum Durability & Low Backpressure

• Provides:

  • Long column lifetime

  • High robustness under routine use

  • Lower backpressure than smaller particle sizes

✅ Ideal for:

• Preparative method scouting

• High sample loads and routine workflows

• Systems with pressure limitations

Wide pH Stability (pH 2–12) – Extreme Method Capability

• Designed for acidic and basic mobile phase conditions

• Suitable for:

  • Strong acids

  • Strong bases

• Maintains stability under aggressive analytical environments

✅ Enables method development beyond standard C18 limitations

Why Choose RP C18 ABX™ Columns (5 µm)

Extreme pH Ruggedness

• Stable across full pH range (2–12)

• Ideal for challenging analytes and harsh methods

Maximum Robustness & Method Transferability

• Designed for long column life and reproducibility

• Ideal for method transfer across laboratories

Versatile Analytical Performance

• Suitable for:

  • Hydrophobic compounds

  • Neutral analytes

  • Long‑chain fatty acids

✅ One of the most popular ABX columns for routine and preparative‑scouting workflows

Preparative Scouting Advantage

• Commonly used to:

  • Develop scalable purification methods

  • Transition from analytical to preparative chromatography

• Supports:

  • Method transfer and scale‑up optimization

✅ Ideal bridge between analytical method development and preparative workflows

System & Sample Considerations

• Compatible with standard HPLC systems

• Suitable for routine analytical and method development workflows

Recommended sample preparation:

• 👉 Use 0.2 µm syringe filters for proper sample preparation before injection

✅ Helps:

• Prevent column fouling

• Maintain long‑term performance and reproducibility

Typical Applications

• HPLC analytical separations

• Acidic and basic compound analysis

• Pharmaceutical method development

• Stability‑indicating methods

• Preparative method scouting

• Routine QC workflows

Important Usage Notes

• Designed for standard analytical HPLC systems (4.6 mm ID)

• Suitable for extreme pH mobile phases (pH 2–12)

• Ideal for fast screening and method development

• Preferred when robustness and scalability are prioritized